Institute of Aquaculture

Genetics and Reproduction Research Group

My main areas of scientific interest are on genetics of aquatic organisms of relevance to aquaculture, including: sex determination and its control; genetic management and selective breeding; chromosome set manipulations and clonal lines; genomics; cytogenetics; applications of molecular markers; transgenic fish. Current research is on sex determination, genetics of colour and genomics in tilapias, genetic management and selective breeding in species including carps and cod.

I have worked in a variety of countries over the last two decades, with a focus on SE and S Asia, mostly through projects, PhD students and consultancy on the genetics of carps. I am a member of the editorial board of Aquaculture, an adviser to the International Foundation for Science, and have served as an adviser to the Commonwealth Scholarship Commission and as a board member of the International Association for Genetics in Aquaculture.

I teach on modules relating to aquaculture and aquatic genetics as part of the MSc programmes in Sustainable Aquaculture, Aquatic Veterinary Studies/Aquatic Pathobiology and Aquatic Resource Development, and to BSc students in 3rd and 4th years, doing Honours in Aquaculture or Marine Biology. This involves both face-to-face teaching and distance learning.

Selected Recent Publications

Campos-Ramos R, Harvey SC, Penman DJ (2009). Sex-specific differences in the synaptonemal complex in the genus Oreochromis (Cichlidae). Genetica 135:325-332.

Ocalewicz K, Mota-Velasco JC, Campos-Ramos R, Penman DJ (2008) FISH and DAPI staining of the synaptonemal complex of the Nile tilapia (Oreochromis niloticus) allow orientation of the unpaired region of bivalent 1 observed during early pachytene, Chromosome Research 16:1062 (abstract only).

Basavaraju Y, Mohan Kumar HM, Pradeep Kumar S, Umesha D, Srivastava PP, Penman DJ, Mair GC (2008). Production of genetically female common carp, Cyprinus carpio, through sex reversal and progeny testing. Asian Fisheries Science 21:355-368.

Penman DJ, Piferrer F (2008). Fish gonadogenesis. Part 1. Genetic and environmental mechanisms of sex determination. Reviews in Fisheries Science 16(S1):14-32.

Ocalewicz K, Penman DJ, Babiak I (2008). Variation in size and location of the Ag-NOR in the Atlantic halibut (Hippoglossus hippoglossus). Genetica 133:261-267.

Cnaani A, Lee B-Y, Zilberman N, Ozouf-Costaz C, Hulata G, Ron M, D’Hont A, Baroiller J-F, D’Cotta H, Penman DJ, Tomasino E, Coutanceau J-P, Pepey E, Shirak A, Kocher, TD (2008). Genetics of sex determination in tilapiine species. Sexual Development 2:43-54.

Martinez-Chavez CC, Al-Khamees S, Campos-Mendoza A, Penman DJ, Migaud H (2008) Clock-controlled endogenous melatonin rhythms in Nile tilapia (Oreochromis niloticus niloticus) and African catfish (Clarias gariepinus). Chronobiology International 25:31-49.

Herlin M, Delghandi M, Wesmajervi M, Taggart JB, McAndrew BJ, Penman DJ (2008). Analysis of the parental contribution to a group of fry from a single day of spawning from a commercial Atlantic cod (Gadus morhua) breeding tank. Aquaculture 274:218-224.

Herlin M, Taggart JB, McAndrew BJ, Penman DJ (2007). Parentage allocation in a complex situation: a large commercial Atlantic cod (Gadus morhua) mass spawning tank. Aquaculture 272S1:S195-S203.

Basavaraju Y, Prasad DT, Rani K, Pradeep Kumar S, Naika UD, Jahageerdar S, Srivastava PP, Penman DJ and Mair GC (2007). Genetic diversity in common carp stocks assayed by random-amplified polymorphic DNA markers. Aquaculture Research 38:147-155.

Gheyas AA, Cairney M, Gilmour AE, Sattar MA, Das TK, McAndrew BJ, Penman DJ and Taggart JB (2006). Characterization of microsatellite loci in silver carp (Hypophthalmichthys molitrix) and cross-amplification in other cyprinid species. Molecular Ecology Notes 6:656-659.

Penman, DJ (2006). Potential genetic impacts of aquaculture. In: Aquaculture Compendium, CAB International, Wallingford, UK (http://www.cabi.org/compendia/ac/index.asp).

Mia MY, Taggart JB, Gilmour AE, Gheyas AA, Das TK, Kohinoor AHM, Rahman MA, Sattar MA, Hussain MG, Mazid MA Penman DJ, McAndrew BJ (2005). Detection of hybridization between Chinese carp species (Hypophthalmichthys molitrix and Aristichthys nobilis) in hatchery broodstock in Bangladesh, using DNA microsatellite loci. Aquaculture 247:267-273.

Penman, DJ, Gupta, MV and Dey, MM (eds) (2005). Carp Genetic Resources for Aquaculture in Asia, WorldFish Center Technical Report 65. WorldFish Center, Penang, Malaysia. 152p. ISBN 983-2346-35-5.

Ezaz, MT, Harvey, SC, Boonphakdee, C, Teale, AJ, McAndrew, BJ and Penman, DJ (2004). Isolation and physical mapping of sex-linked AFLP markers in the Nile tilapia (Oreochromis niloticus L.). Marine Biotechnology 6:435-445.

Ezaz, MT, Sayeed, S, McAndrew, BJ and Penman, DJ (2004). Use of microsatellite loci and AFLP markers to verify gynogenesis and clonal lines in Nile tilapia Oreochromis niloticus L. Aquaculture Research 35:1472-1481.

McAndrew, BJ, Penman, DJ, Frank-Lawale, A, Brown, C, Ureta Schmidt, J and Herlin, M (2004). Applying genetics in aquaculture. Fish Farmer 27(4):10-13.

Basavaraju, Y, Penman, DJ and Mair, GC (2004). Handbook on Genetic Management of Carps: a Guide to the Theoretical and Practical Aspects of Genetic Management of Carps in Hatcheries. University of Agricultural Sciences, Bangalore, India. 28p.

Karayücel, I, Ezaz, MT, Karayücel, S, McAndrew, BJ and Penman, DJ (2004). Evidence for two unlinked sex reversal loci in the Nile tilapia, Oreochromis niloticus, and for linkage of one of these to the red body colour gene. Aquaculture 234:51-63.

Ezaz, MT, Myers, JM, Powell, SF, McAndrew, BJ and Penman, DJ (2004). Sex ratios in the progeny of androgenetic and gynogenetic YY male Nile tilapia, Oreochromis niloticus L., Aquaculture 232:205-214.

Ezaz, MT, McAndrew, BJ and Penman, DJ (2004). Spontaneous diploidization of the maternal chromosome set in Nile tilapia (Oreochromis niloticus L.) eggs. Aquaculture Research 35:271-277.

Harvey, SC, Boonphakdee, C, Campos-Ramos, R, Ezaz, MT, Griffin, DK, Bromage, NR and Penman, DJ (2003). Analysis of repetitive DNA sequences in the sex chromosomes of Oreochromis niloticus, Cytogenetics and Genome Research (special issue on Sex Determination in Vertebrates) 101:314-319.

Lee, B-Y, Penman, DJ and Kocher, TD (2003). Identification of a sex-determining region in Nile tilapia (Oreochromis niloticus) using bulked segregant analysis. Animal Genetics 34:379-383.

Basavaraju, Y, Penman, DJ and Mair, GC (2003). Stock evaluation and development of a breeding programme for common carp (Cyprinus carpio) in Karnataka, India: progress of a research project. NAGA, The WorldFish Center Quarterly 26(2):30-33.

Karayücel, I, Penman, D, Karayücel, S and McAndrew, B (2003). Thermal and hormonal feminization of all male YY Nile tilapia, Oreochromis niloticus L. Israeli J Aquaculture 55:114-122.

Bostock, JC, Telfer, TC, McAndrew, B, Penman, D, Young, J and Muir, J (2003). The potential impact of technological innovation on the aquaculture industry. A Report to the Royal Commission on Environmental Pollution, London. 84p.

Campos-Ramos, R, Harvey, SC, McAndrew, BJ and Penman DJ (2003). An investigation of sex determination in the Mozambique tilapia, Oreochromis mossambicus, using synaptonemal complex analysis, FISH, sex reversal and gynogenesis. Aquaculture 221:125-140.

Harvey, SC, Kwon, JY and Penman, DJ (2003). Physical mapping of the brain and ovarian aromatase genes in the Nile tilapia, Oreochromis niloticus, by fluorescence in situ hybridisation. Animal Genetics 34:62-64.

Griffin, DK, Harvey, SC, Campos-Ramos, R, Ayling, L-J, Bromage, NR, Masabanda, J and Penman, DJ (2002). Early origins of the X and Y chromosomes: lessons from tilapia. Cytogenetics and Genome Research 99:157-163.

Karayücel, S, Karayücel, I, Penman, D and McAndrew, B (2002). Production of androgenetic Nile tilapia, Oreochromis niloticus L.: optimization of heat shock duration and application time to induce diploidy. Israeli J Aquaculture 54:145-156.

Harvey, SC, Masabanda, J, Carrasco, LAP, Bromage, NR, Penman, DJ and Griffin, DK (2002). Molecular-cytogenetic analysis reveals sequence differences between the sex chromosomes in Oreochromis niloticus: evidence for an early stage of sex chromosome evolution. Cytogenet Genome Research 97:76-80.

Harvey, SC, Campos-Ramos, R, Kennedy, DD, Ezaz, MT, Bromage, NR, Griffin, DK and Penman, DJ (2002). Karyotype evolution in tilapia: mitotic and meiotic chromosome analysis of Oreochromis karongae and O. niloticus x O. karongae hybrids. Genetica 115:169-177.

Penman, DJ, Hussain, MG, McAndrew, BJ and Mazid, MA (eds) (2002). Genetic Management and Improvement of Exotic Carp Species in Bangladesh: Proceedings of a DFID-AFGRP/BFRI Workshop, 12-14 Feb 2002, Dhaka, Bangladesh. BFRI, Mymensingh, Bangladesh. 123p. ISBN 984-32-0288-0.

Harvey, SC, Powell, SF, Kennedy, DD, McAndrew, BJ and Penman, DJ (2002). Karyotype analysis of Oreochromis mortimeri and Sarotherodon melanotheron. Aquaculture Research 33:339-342.

Hilsdorf, AW, Penman, DJ, Cunha Farias, E and McAndrew, BJ (2002). Melanophore appearance in wild and red tilapia embryos. Pigment Cell Res 15:57-61.

Kwon, JY, McAndrew, BJ and Penman, DJ (2002). Inhibition of aromatase activity suppresses high-temperature feminisation of genetic male (YY) Nile tilapia, Oreochromis niloticus. J Fish Biol 60:625-636.

Basavaraju, Y, Mair, GC, Mohan Kumar, HM, Pradeep Kumar, S, Keshavappa, GY and Penman, DJ (2002). An evaluation of triploidy as a potential solution to the problem of precocious sexual maturation in common carp, Cyprinus carpio, in Karnataka, India. Aquaculture 204:407-418.

Campos-Ramos, R, Harvey, SC, Masabanda, JS, Carrasco, LAP, Griffin, DK, McAndrew, BJ, Bromage, NR and Penman, DJ (2001). Identification of putative sex chromosomes in the blue tilapia, Oreochromis aureus, through synaptonemal complex and FISH analysis. Genetica 111(1-3):143-153.

Dunham, RA, Majumdar, K, Hallerman, E, Mair, G, Hulata, G, Liu, Z, Pongthana, N, Bakos, J, Penman, D, Gupta, M, Rothlisberg, P and Hoerstgen-Schwark, G (2001). Review of the status of aquaculture genetics. In: Subasinghe, RP, Bueno, P, Phillips, MJ, Hough, C, McGladdery, SE and Arthur, JR (eds). Aquaculture in the Third Millenium. Technical Proceedings of the Conference on Aquaculture in the Third Millenium, Bangkok, Thailand, 20-25 February 2000. NACA, Bangkok and FAO, Rome. pp. 129-157.

Rahman, MA, Ronyai, A, Engidaw, BZ, Jauncey, K, Hwang, GL, Smith, A, Roderick, EE, Penman, DJ, Varadi, L and Maclean, N (2001). Growth and nutritional trials on transgenic Nile tilapia containing an exogenous fish growth hormone gene. J Fish Biol 59:62-78.

Kwon, JY, McAndrew, BJ and Penman, DJ (2001). Cloning of brain aromatase gene and expression of brain and ovarian aromatase genes during sexual differentiation in genetic male and female Nile tilapia Oreochromis niloticus. Mol Reprod Dev 59:359-370.

Sarder, MRI, Thompson, KD, Penman, DJ and McAndrew, BJ (2001). Immune responses of Nile tilapia (Oreochromis niloticus L.) clones: I. Non-specific responses. Dev Comp Immunol 25:37-46.

Kwon, JY, Haghpanah, V, Kogson-Hurtado, LM, McAndrew, BJ and Penman, DJ (2000). Masculinisation of genetic female Nile tilapia (Oreochromis niloticus) by dietary administration of an aromatase inhibitor during sexual differentiation. J. Exp. Zool. 287:46-53.

Penman, DJ and McAndrew, BJ (2000). Genetics for the management and improvement of cultured tilapias. In: Beveridge, MCM and McAndrew, BJ (eds). Tilapias: biology and exploitation. Kluwer, Dordrecht, the Netherlands. pp. 227-266.